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PCR protocols for the detection of swine influenza

'Perfect match' matrix (M) gene RRT PCR

Validation has shown that this modified M gene RRT PCR assay can sensitively detect

• current influenza A viruses known to infect pigs in Eurasia, and
• can also detect current pandemic (H1N1) 2009 virus in pigs infected experimentally.

Full protocol for 'perfect match' matrix (M) gene RRT PCR for influenza A in pigs

H1-118 RRT PCR

Validation has shown that this RRT PCR assay differentiates the pandemic (H1N1) 2009 influenza virus in a European swine setting.

• It has been shown to detect current pandemic (H1N1) 2009 influenza virus in pigs infected (i) experimentally and (ii) in the field.
• Other established European swine influenza viruses (SIVs) of subtypes H1N1, H1N2 and H3N2 are not detected by this assay.
• It may also be applied to investigation of suspect pandemic (H1N1) 2009 influenza infection in poultry.

See the full protocol for H1-118 RRT PCR for pandemic (H1N1) influenza A

Any technical enquiries about this protocol should be sent to: aiwrl@vla.defra.gsi.gov.uk

For information on the PCR protocols for the detection of avian influenza, see our web pages about the VLA International Reference Laboratory for avian influenza.